Rendering different with audio interface?

hey guys I was wondering if there is a difference in cubase render quality if I have an audio interface plugged in vs if I have nothing plugged in at all and just going off the stock asio?

for example: if I render a cubase track with my RME UCX plugged in vs if I dont have it plugged in to my computer and just going off my laptop speakers? is there any difference in render quality inside cubase?

No. There is no audio software that would utilize the D/A converters of your audio interface when processing audio in any form. Your audio interface only handles what happens when audio leaves your DAW.

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