3D analyzer after master section

Is this possible without rendering first? For example using 3D analyzer I see a spike 255 hertz. Using a EQ on the master section lowering 255 with a high Q. Now using the 3D analyzer again nothing is different. Please help.

Rendering is the process of creating the modified sound file; if you don’t render you don’t create it, only hear what it will be like when rendered.

Paul