Variaudio stops working when lowering volume on clip?

Hi friends! So i have a bass recording as one file in a song. I have done some variaudio tuning with great results.

But when i listened to the track today the bass is out of tune in some areas. I could not understand why but i just remembered that on this particular song i have lowered the volume on the track. Left click on the track and then lowered the volume a couple of DBs.

Would lowering the volume cause variaudio to stop working since the track has been “altered”?

To be more clear: I have lowered the volume on the track AFTER applying variaudio.